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排序方式: 共有183条查询结果,搜索用时 15 毫秒
31.
Lecchi C Avallone G Giurovich M Roccabianca P Ceciliani F 《Veterinary journal (London, England : 1997)》2009,180(2):256-258
Combined quantitative (real-time) polymerase chain reaction and immunohistochemistry were used to evaluate the expression of the minor acute phase protein alpha 1-acid glycoprotein (AGP, orosomucoid) in bovine extrahepatic tissues. AGP was produced mainly in the salivary glands and spleen, whereas minor expression was detected in all other tissues sampled, including lung, lymph nodes, uterus, ovary, kidney and tongue. The findings were consistent with immunohistochemical results. In view of the immunomodulatory and direct antibacterial activity of AGP, its expression in the salivary glands may signal an involvement in the regulation of the local immunity, even in non-pathological conditions. 相似文献
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Elena Testani Gabriele Campanelli Fabrizio Leteo Corrado Ciaccia Stefano Canali Fabio Tittarelli 《Compost science & utilization》2017,25(2):112-119
In the past decade, the increasing environmental concerns regarding the use of non-renewable resources, such as peat, for growing media production has led to a search for alternative materials as substrate components. Until now, compost represents the most investigated one. Despite the critical aspects related to compost use (i.e., its high pH, salinity, and potential content of toxic elements), partial peat substitution with this material represents an achievable objective. In this study, green compost was evaluated as a component of peat substrates for organic sweet pepper (Capsicum annuum L.) seedling production. Three compost rates (CR) were compared: 0 (peat without compost), 30, and 60% of compost v/v. Two pepper cultivars, one derived from a local (medium-Adriatic) germplasm (L) and a commercial hybrid (H), were tested. Furthermore, organic fertilizers based on hydrolyzed proteins, of animal (A) and vegetal (V) origin, were compared. Substrates were analyzed for characterization (pH, EC, nutrient contents, heavy metals). Seedling performances were evaluated in terms of size, biomass, nutrient uptake, and toxic element contents. Indices able to assess seedling resistance to transplant stress were also calculated. Results showed that CR60 treatment was able to produce seedlings with the same biomass of control. CR30, instead, assured the same chance to resist the transplanting stress of CR0. The performances of fertilizer A were significantly higher than those of fertilizer V. Local germplasm (L) showed the highest resistance to transplant stress, while H was more performing in nitrogen uptake. The risk of seedling contamination by toxic elements resulted negligible. 相似文献
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Passamonti F Veronesi F Cappelli K Capomaccio S Coppola G Marenzoni ML Piergili FD Verini SA Coletti M 《Comparative immunology, microbiology and infectious diseases》2010,33(1):73-83
Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, affects several species of wild and domesticated mammals, including horses. In this work we compared direct and indirect methods to evaluate A. phagocytophilum presence in Central Italy: 135 sera were screened by IFA for A. phagocytophilum and other haemopathogens (Theileria equi and Babesia caballi). Each horse was also tested for A. phagocytophilum 16S rRNA with a nested-PCR technique. In order to examine the risk of A. phagocytophilum transmission, 114 ticks were examined for the presence of A. phagocytophilum by PCR targeting the 16S rRNA. The seroprevalence against A. phagocytophilum was 17.03% and 11 horses (8.14%) showed positive PCR results. The concordance rate of A. phagocytophilum detection between IFAT and PCR had a K value of 0.34. 相似文献
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Ángeles G. Mayor Sonia Kéfi Susana Bautista Francisco Rodríguez Fabrizio Cartení Max Rietkerk 《Landscape Ecology》2013,28(5):931-942
Conceptual frameworks of dryland degradation commonly include ecohydrological feedbacks between landscape spatial organization and resource loss, so that decreasing cover and size of vegetation patches result in higher water and soil losses, which lead to further vegetation loss. However, the impacts of these feedbacks on dryland dynamics in response to external stress have barely been tested. Using a spatially-explicit model, we represented feedbacks between vegetation pattern and landscape resource loss by establishing a negative dependence of plant establishment on the connectivity of runoff-source areas (e.g., bare soils). We assessed the impact of various feedback strengths on the response of dryland ecosystems to changing external conditions. In general, for a given external pressure, these connectivity-mediated feedbacks decrease vegetation cover at equilibrium, which indicates a decrease in ecosystem resistance. Along a gradient of gradual increase of environmental pressure (e.g., aridity), the connectivity-mediated feedbacks decrease the amount of pressure required to cause a critical shift to a degraded state (ecosystem resilience). If environmental conditions improve, these feedbacks increase the pressure release needed to achieve the ecosystem recovery (restoration potential). The impact of these feedbacks on dryland response to external stress is markedly non-linear, which relies on the non-linear negative relationship between bare-soil connectivity and vegetation cover. Modelling studies on dryland vegetation dynamics not accounting for the connectivity-mediated feedbacks studied here may overestimate the resistance, resilience and restoration potential of drylands in response to environmental and human pressures. Our results also suggest that changes in vegetation pattern and associated hydrological connectivity may be more informative early-warning indicators of dryland degradation than changes in vegetation cover. 相似文献
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F Torchio S Giacosa SR Segade F Mattivi V Gerbi L Rolle 《Journal of agricultural and food chemistry》2012,60(36):9006-9016
An instrumental texture analysis method has been optimized for the differentiation of grape ripening stages based on the simultaneous determination of mechanical and acoustic parameters of the seeds. Two factorial central composite design was used to optimize the most influencing operative conditions (speed and deformation) on mechanical and acoustic measurements. This experimental design in combination with response surface methodology showed that the most responsive parameters to changes in seeds during ripening were Young's modulus of elasticity, many acoustic parameters measured with instrumental gain set to 0 (acoustic energy, linear distance, and number of peaks) and others measured at 24 dB gain (linear distance, number of peaks, and average pressure level). However, the optimal operative conditions depended on the texture parameter used. A correlation study between texture parameters and phenolic compounds of the seeds revealed that acoustic parameters like the average pressure level could be proposed as phenolic maturity indices. 相似文献
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Fabrizio Scartezzini Pietro Fusani Nicola Aiello Carla Vender 《Genetic Resources and Crop Evolution》2012,59(3):465-471
Alpine blue-sow-thistle (Cicerbita alpina (L.) Wallr.) is a perennial herb distributed all along the entire Alpine Arc, the shoots of which are still collected in
the wild in several areas of Northern-Eastern Italy for food purposes. Our paper deals with experiments of domestication of
this species carried out in the surroundings of Trento and lasted from 2004 to 2010. The cultivation was carried out transplanting
seedlings, obtained by wild seed of a unique accession. One experimental trial (2004/2008) was carried in the location Frisanchi,
located at 1,078 m a.s.l. In this trial the theses compared 4 harvests, 1 week spaced, carried out respectively in the 2nd,
3rd and 4th year after the transplant. Of the total quantity harvested after 4 years, the most shoots (60) and the highest
dried weight per m2 (14 g) were obtained from shoots collected 2 or 3 years after being transplanted. However, comparing the shoots yielded in
the last year of the trial only (2008), the best results were obtained from 3 or 4-year-old transplants (42 shoots/m2; 8.2 g dry weight). Nevertheless there four times harvesting of shoots weakened the plants’ development and a high failure
rates was recorded. In addition to the real experimental trial another small cultivation was carried out in an higher location,
Monte Bondone at 1,500 m a.s.l., in which 5 years after the transplant (2010) only 2 harvests were carried out. In this experiment
the yield recorded was very encouraging (10.5 g m2) and also the quality of the shoots collected was good. 相似文献
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